NAD+
Nicotinamide Adenine Dinucleotide (Oxidized Form) — Redox Coenzyme
Nicotinamide adenine dinucleotide (NAD⁺) is a dinucleotide coenzyme functioning as an obligate hydride-transfer redox partner in oxidative phosphorylation, TCA cycle reactions, and fatty acid β-oxidation. It serves as the rate-limiting substrate for sirtuins, poly-ADP-ribose polymerases (PARPs), CD38/CD157 glycohydrolases, and SARM1 NAD⁺ glycohydrolase. Experimental applications span enzyme kinetics, mitochondrial respirometry, PARP trap assays, sirtuin biochemistry, intracellular NAD⁺/NADH ratio profiling, and transcriptomic studies of metabolic senescence pathways.
| PROPERTIES | |
|---|---|
| Molecular Formula | C21H27N7O14P2 |
| Molecular Weight (g/mol) | 663.4 |
| Monoisotopic Mass (Da) | 663.1091 |
| Topological Polar Surface Area (Ų) | 321 |
| Complexity | 1120 |
| XLogP (octanol-water partition) | -6 |
| Heavy Atom Count | 44 |
| H-Bond Donor Count | 7 |
| H-Bond Acceptor Count | 18 |
| Rotatable Bond Count | 11 |
| InChIKey | BAWFJGJZGIEFAR-NNYOXOHSSA-N |
| PubChem CID | 5892 |
All values above are taken from PubChem CID 5892 and can be checked against that record.
2D Structure
| CHEMICAL IDENTIFIERS | |
|---|---|
| CAS Number | 53-84-9 |
| PubChem CID | 5892 |
| IUPAC Name | [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2R,3S,4R,5R)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate |
| InChIKey | BAWFJGJZGIEFAR-NNYOXOHSSA-N |
| InChI | InChI=1S/C21H27N7O14P2/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33/h1-4,7-8,10-11,13-16,20-21,29-32H,5-6H2,(H5-,22,23,24,25,33,34,35,36,37)/t10-,11-,13-,14-,15-,16-,20-,21-/m1/s1 |
| SMILES | C1=CC(=C[N+](=C1)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)([O-])OP(=O)(O)OC[C@@H]3[C@H]([C@H]([C@@H](O3)N4C=NC5=C(N=CN=C54)N)O)O)O)O)C(=O)N |
Identifiers retrieved from PubChem CID 5892.
Certificate of analysis Sample lot
NAD+
- Lot
- MLP-NAD-20260126
- SKU
- GD-NAD
- Manufactured
- 26 Aug 2026
- Retest
- 25 Nov 2028
Reported composition each test reported separately, not a mass balance
Every figure is the result printed on the certificate. Purity is by HPLC area integration and the others are separate determinations on the same lot, so they are shown as separate bars rather than as shares of one total.
Test results as reported
| # | Test | Specification | Result |
|---|---|---|---|
| 1 | Appearance | White to yellowish powder or crystalline powder | Conforms |
| 2 | Identification | Conform with NMR | Conforms |
| 3 | Na content (ICP-OES) | ≤0.5% | 0.003% |
| 4 | Water | ≤10.0% | 6.1% |
| 5 | pH | 2.0–4.0 | 2.3 |
| 6 | Purity (HPLC) | ≥98.5% | 99.56% |
| 7 | Assay | ≥97.0% | 99.29% |
| 8 | Heavy metals: lead (Pb) | ≤0.5 ppm | <0.5 ppm |
| 9 | Heavy metals: arsenic (As) | ≤0.5 ppm | <0.5 ppm |
| 10 | Heavy metals: cadmium (Cd) | ≤0.5 ppm | <0.5 ppm |
| 11 | Heavy metals: mercury (Hg) | ≤0.5 ppm | <0.5 ppm |
| 12 | Spectroscopy: A340/A260 | 0.39–0.47 (0.43 ± 0.04) | 0.41 |
| 13 | Spectroscopy: A250/A260 | 0.75–0.91 (0.83 ± 0.08) | 0.84 |
| 14 | Spectroscopy: A280/A260 | 0.19–0.23 (0.21 ± 0.02) | 0.22 |
| 15 | Molar absorptivity ε260 (pH 7.5) | 16.2–19.8 ×10³ L·mol⁻¹·cm⁻¹ (18.0 ± 1.8) | 17.9 ×10³ |
| 16 | Molar absorptivity ε340 (pH 10) | 5.7–6.9 ×10³ L·mol⁻¹·cm⁻¹ (6.3 ± 0.6) | 6.5 ×10³ |
| 17 | Residual solvent: methanol | ≤1.0% | N.D |
| 18 | Residual solvent: ethanol | ≤2.0% | 0.07% |
| 19 | Microbial: total colony count | ≤750 CFU/g | <750 CFU/g |
| 20 | Escherichia coli | <3 MPN/g | N.D |
Identity verified against PubChem, not a measurement of this lot
- CAS number
- 53-84-9
- Molecular formula
- C21H27N7O14P2
- Molecular weight
- 663.43 g/mol
- Monoisotopic mass
- 663.1091 Da
- InChIKey
- BAWFJGJZGIEFAR-NNYOXOHSSA-N
- PubChem CID
- 5892
Corrections and queries 2 corrected, 1 queried with the supplier
| Field | On the supplier document | Here | Why |
|---|---|---|---|
| Assay basis | Assay 99.29% and Water 10.0% max / 6.1% found, with no basis stated | shown as reported, queried | An assay of 99.29% and a water content of 6.1% cannot both be on an as-is basis: they sum to 105.4%. The assay is almost certainly on an anhydrous basis, but the certificate does not say so. A measured result is not ours to rewrite, so both stand as printed and we have asked the supplier to state the basis. |
| Microbial test name | Escherichia Coli | Escherichia coli | Capitalisation of a species name. |
| Batch quantity | 150 kg | not published | The figure is the manufacturer's bulk batch size, not a property of the material, so it is left off the page. |
A measured result is never rewritten. Where a reported figure does not stand up, it is shown as printed and marked as queried.
Sample lot. This is the report supplied with the sample of this compound. A production lot ships with its own report, published here in the same form and indexed by its own lot number.
The certificate specifies storage sealed, dry and away from light: at room temperature within six months, and at 2–8 °C beyond six months.
Every analytical figure above is transcribed from the supplier’s report for lot MLP-NAD-20260126. Identity is the PubChem record, which can be checked independently. Purity is the main peak’s share of integrated peak area at the detection wavelength, so it measures related impurities. Water, salts and counter-ions are not part of it. These are the supplier’s release documents, not independent third-party tests.
This product is sold exclusively for in vitro and laboratory research use. It has not been evaluated by the FDA. Not approved for diagnostic, therapeutic, or veterinary use.
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